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Application Data
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Hamster CD29 Monoclonal Antibody | anti-CD29 antibody

HAMSTER ANTI MOUSE CD29:FITC

Gene Names
Itgb1; CD29; Fnrb; gpIIa; Gm9863; AA409975; AA960159; 4633401G24Rik; ENSMUSG00000051907
Applications
Flow Cytometry, Functional Assay
Synonyms
CD29, Antibody; HAMSTER ANTI MOUSE CD29:FITC; anti-CD29 antibody
Ordering
 
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For Research Use Only!
Host
Hamster
Clonality
Monoclonal
Isotype
IgG
Clone Number
HM beta 1-1
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
798
Applicable Applications for anti-CD29 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Immunogen
Purified mouse VLA-4 antigen
Fusion Partners
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: 18 months from date of despatch.

Application Data

(Staining of mouse peripheral blood lymphocytes with Hamster anti Mouse CD29: Low Endotoxin)

Application Data

(Staining of mouse spleen cells with Hamster anti Mouse CD29: Alexa Fluor 647)

Application Data

(Staining of mouse peripheral blood lymphocytes with Hamster anti Mouse CD29)

Application Data

(Staining of mouse spleen cells with Hamster anti Mouse CD29: Alexa Fluor 488)

Application Data

(Staining of mouse peripheral blood lymphocytes with Hamster anti Mouse CD29:FITC)

Application Data

(Staining of mouse peripheral blood lymphocytes with Hamster anti Mouse CD29)

Related Product Information for anti-CD29 antibody
Hamster anti Mouse CD29 antibody, clone HM beta 1-1 recognizes the murine integrin beta 1 subunit (CD29), a 110kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation. Hamster anti Mouse CD29 antibody, clone HM beta 1-1 is reported to inhibit beta 1 integrin mediated adhesion (Noto et al. 1995).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,700 Da
NCBI Official Full Name
integrin beta-1
NCBI Official Synonym Full Names
integrin beta 1 (fibronectin receptor beta)
NCBI Official Symbol
Itgb1
NCBI Official Synonym Symbols
CD29; Fnrb; gpIIa; Gm9863; AA409975; AA960159; 4633401G24Rik; ENSMUSG00000051907
NCBI Protein Information
integrin beta-1; VLA-4 subunit beta; fibronectin receptor subunit beta
UniProt Protein Name
Integrin beta-1
UniProt Gene Name
Itgb1
UniProt Entry Name
ITB1_MOUSE

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Product Notes

The CD29 itgb1 (Catalog #AAA11872) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD29 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the CD29 itgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD29, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.